Coding

Part:BBa_K3317013:Design

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)


pduU protein from S. enterica LT2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
    Illegal PstI site found at 161
    Illegal AgeI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

described by Yung, M. C et. to 2017

References

Yung, M. C., Bourguet, F. A., Carpenter, T. S., & Coleman, M. A. (2017). Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. Microbial cell factories, 16(1), 71.